A NEW METHOD FOR EMBEDDING TEMPORAL BONE TO STUDY THE EFFECT OF CISPLATIN ON THE INNER EAR OF GUINEA PIGS
|Full paper||Not Available|
This paper reports the pattern of ototoxicity of cisplatin on pigs using a new technique for preparing undeclactftd bone sections by embedding the cochlea in Methyl methacrylate (MM/U. Forty-four guinea pigs were divided into a control group (8 animals) and 2 study groups (group I and group II), each comprised 18 animals. The animals of group I (acute treated group) were injected with 2 intraperitoneal (LP) injections of cisplatin (7.5 mg / kg / day) separated by interval of 5 days (total dose 15 mg / kg) i.e., a dose largely exceeding the LD 50 dose (9.7 mg/ kg by a single I.P. injection) and the animals of group II (chronic treated group) were injected by a dose (1.5 mg/kg/ day) for 10 consecutive days (total dose 15 mg /kg). Results showed that the acute treated group was affected by degenerative changes in the organ of corn, stria vascularis, spiral ganglion cells more than chronic treated group and the affection was more prominent at basal turns more than middle and apical turns. Also the results showed that the new method for preparing undecalcified specimens by embedding in MMA had an upper hand in demonstration of fine details of delicate structures of the cochleae more than traditional method which depends on decalcification of bone.