THE EFFECT OF HYDROXOCOEtALAMIN ON NITRIC OXIDE MEDIATED RELAXATIONS IN RABBIT AORTA
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This work was done to investigate the effect of hydroxocobalamin on nitric oxide mediated relaxation of aortic strips of rabbit. We investigate the effect of hydroxocobalamin on rabbit aortic strips with intact endothelium and rabbit aortic strips with denuded endothelium. In rabbit aortic rings, hydroxocobalamin (30μM) produced concentration dependent reduction of the relaxant action of nitrie oxide (NO) in rabbit aortic rings with or without endothelium. Hydroxocobalarnin (30μM) had no effect in submaximal relaxation elicited by papaverine in aortic rings. Hydroxocobalarnin (101.4.M) produced concentration dependent reduction of the relaxant action of acetylcholine which is mediated through the endothelium dependent nitric oxide. A concentration of (30μM), Hydroxocobalarnin has been abolished the responses to acetylcholine. From this study we can detect that hydroxocobalamin reduces responses to (NO) in rabbit aortic rings by sequesting nitric oxide so that the concentration of (NO) available for activating soluble guanylate-cyclase enzyme relaxing the smooth muscle is reduced. In conclusion, hydroxocobalamin may serve as an additional tool for investigating nitric oxide mediated physiological processes.