EFFECT OF CHLOROQUINE ON INSULIN RESISTANCE INDUCED BY HIGH FAT DIET IN RATS
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Thirty albino rats weighing from 100 to 150 gm were randomly divided into 3 groups, the first group fed standard balanced diet, the second group fed high fat diet and the third group fed high fat diet but in addition each rat in this group received chloroquine in a daily dose of 90mg/kg orally. After 8 weeks of these dietary regimens, the rats were decapitated and soleus muscle and epididymal or parametrial fat were taken from all animals in each group. Both basal and insulin stimulated glucose uptake was estimated in every tissue . The levels of glucose, insulin, total cholesterol, triglycerides, high density lipoprotein (HDL) cholesterol and low density lipoprotein (LDL) cholesterol in the serum of each rat were also evaluated. Results of the present work showed that persistant intake of fat rich diet significantly reduced the basal and insulin stimulated glucose uptake by both muscle and fat tissues. Furthermore, there were also significant increases in the fasting serum concentrations of glucose, total cholesterol, triglycerides and LDL . On the other hand, significant reductions in the levels of fasting serum insulin and HDL were also observed. Chloroquine administration significantly abolished the inhibitory effect of high fat diet on glucose uptake. It also induced significant decreases in serum concentrations of glucose, total cholesterol, triglycerides and LDL as well as significant increases in serum levels of insulin and HDL . In conclusion, the persistant intake of high fat diet reduces glucose utilization and participates in hyperglycemia and dyslipidemia Chloroquine increases muscle and fat tissue insulin responsiveness as well as it abolishes dyslipidemia and subsequently can have favourable effects on insulin resistance . Accordingly, chloroquine may have a therapeutic potential in the treatment of diabetes mellitus.