The Protective Effect of Vitamin D on Cerebral Infarction in Rats Received High Fructose Diet
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Background and aim: The relationship between insulin resistance (IR), hypovitamine D and cerebral infarction and its exact mechanism are not fully understood. However, oxidative stress and proinflammatory mediators may be involved. Thus, the aim of the present study was to investigate the effects of vitamin D on cerebral infarction, insulin resistance and inflammatory mediators in rats received high-fructose-diet. Material and methods: Eight four adult albino rats were divided randomly into 3 groups, normal control group, diabetic group rats received high fructose diet for 2 months without no treatment, diabetic rats received alphacalcidol (10pg//kg/day, orally), which was continued daily throughout the experiment, After 2 months, fasting blood glucose level and insulin, IR was evaluated by the homeostasis model assessment method (HOMA-IR). Some cerebrovaseular risk markers as lipid profile (total cholesterol, IIDL-C and triglycerides), as well as inflammatory biomarker interleukin-6 and cerebral infarction size Were measured. Results: Rats had high fructose diet showed low 1, 25 (OH)2 D, with a significant (p <0.05) increase in fasting blood glucose level and higher HOMA-IR index, total cholesterol, low-density lipoprotein cholesterol (LDL-C), triglyeeride and IL-6 as compared to control group. There were significant correlations between 1, 25 (OH)2 D levels and HOMA-IR index (r=-0.47; p <0.01), I, 25 (OH)2 D, total cholesterol (1=-0.34; p <0.01); HDL-C (r=0.54; p <0.01), LDL-C(R---0.34; P <0.01), IL-6 (r--- 0.33; p <0.05). A two month oral al phacalcidol (10 ug/kg/day, orally) treatment markedly decreased HOMA-IR index (p <0.001), LDL, triglycereides and I1-6 and significantly reduced total cholesterol and cerebral infarction size.