SALMONELLA TYPHI ANTIGENS POSSIBLE CROSS REACTIVE ANTIGENIC EPITOPES WITH OTHERS FROM BRUCELLA AND SHIGELLA SPECIES
|Full paper||Not Available|
The present study was undertaken to determine the possible antigenic cross reactivity between four freshly isolated S.typhi strains with (LPS antigen) with brucella and shigella antisera. These were done by testing S.typhi positive LPS antigen against antisera of brucella abort us, brucella meltinesis, shigella sonnei (phase 1 & 2), shigella jlexeneri polyvalent (1-6, X &I9, shigella boydii polyvalent (I & 2 and 3) and shigella dysenteriae using immunoblotting and EL1SA methods. The S.typhi strains were also tested for their ability to invade Henle 407 human intestinal cell lines and to test their cytotoxic effects on these cell lines. The results obtained showed that there was no cross reactivity between crude LPS antigen, of S.typhi and brucella abortus and meltinesis, but cross reactivity was observed between these antigens and shigella sonnei and flexeneri. Shigella dyesenteriae showed no cross reactivity with LPS of S.typhi. The invasiveness of S.typhi to the Henle 407 human intestinal cell lines insure the major role of invasiveness of S.typhi in the pathogenesis of S.typhic rather than the cytotoxic role in this process.