DOWN REGULATING EFFECT OF IL-4 & IL-10 ON INTERFERON-y IN ATOPIC CHILDREN
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Bronchial asthma and atojiic • dermatitis are chronic inflammatory diseases characterized by increased IgE synthesis as a result of T-cell dysregulation in particular T112 that synthesize IL-4, IL-10 which are responsible for IgE antibody response. This study aimed to asses the levels of IL-4, IL-10, 1FN-y and IgE and their role in the pathogenesis of bronchial asthma and atopic dermatitis bind their correlations with the allergic symptoms severityj. Four groups of children were studied: group I included 10 children with atopic dermatitis, group 11 15 children with mild asthma, group III 15 children with severe asthma and group IV 15 healthy control subjects. In this study IL-4, IL-10 and IFN-y level were estimated from cultured supernatant of peripheral blood mononuclear cells PIINICs by commercial ELISA technique and the level of serum IgE was measured by ELISA. Increased level of IL-4, IL-10 and IgE were demonstrated in atopic dermatitis (143.3±12.7, 116.78±14.67, 715.2±317.9), mild asthma (161.2±14.82, 122.33±13.88, 749.19±320.59) and severe asthmatics (201.47±79.6, 167±24.85,2160.9±1411.7) (P<0.002 and <0.001) while the level of IFN-y decreased (71.4± 16.69, 61.2± 18.23, 42.33± 23.29, respectively) (P>0.001). It is concluded that cytokine analysis is a useful tool to asses the severity of disease, this provides a rationale for the use of IFN-y as therapeutic agent in the line of treatment.