THE SERUM PARAOXONASE ACTIVITY IN PATIENTS WITH CHRONIC RENAL FAILURE ON MAINTENANCE HEMODIALYSIS
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Paraoxonase (POND is a high-density lipoprotein (J-1D4)-associated enzyme that has been shown to reduce the susceptibility of low-density lipoprotein (LDL) to lipid peroxidation. This study aimed to investigate the activity and phenotype distribution of senwi PONI in uremic patients and to evaluate the correlations of uremia-associated substances (urea, crewinine and uric acid) with PON1 activity. This study was carried out on 50 ramie patients on maintenance hemodialysis (HD) for less than one year (1 month — I year), and 50 uremic patients on long-term HD (5-8 years). Fifty age and sex matched apparently healthy individuals were also recruited as a control group. Parvoxon was used as a substrate for measuring basal or sodium chloride-stimulated (Naastimulated)paraaronase activity and phenylacelate was used as a substratefir measuring arylesterase activity. The double substrate method was tiled to assign phenotypes. Serum cholesterol, triglycerides, high-density lipoprotein cholesterol, (HDL-c), low-density lipoprotein cholesterol (LDL-c), urea, creatinine and uric acid were also determined by routine laboraiwy methods. Enzyme activities are expressed as international units per liter of serum or per mmol of HDL-cholesterol (HDL-standardized activities), The results of the present study revealed that basal and NaCI-stimulated paraaronase activity, as well as wylesterase activity expressed per serum volume were significandy lower in hemodialyzed uremic patients computed to the commis (P<0.001). However basal and NaCI-stimulated paraoxonase activity standardized for HDL-c concentrations were not significantly reduced in the bemodialyzed airline patients as compared to controls (P>0.05), contra°, to wylesterase activity which remained significantly lower in patients. Pararinonase phenotype distribution was not different among the three studied groups according to the double substrate method. Serum paratnonase activity correlated inversely with serum urea and oratinine levels, In conclusion, the results of the present surety revealed that uremic patients on maintenance hemodialysis have decreased paraommase/arylesterase activity which might indicate a greater risk of premature atherogenesis.