A POSSIBLE PROTECTIVE EFFECTS OF THE CALCIUM CHANNEL BLOCICER (LACIDIPINE) IN CYCLOSPORINE INDUCED NEPHROTOXICITY IN ADULT ALBINO RATS
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ABSTRACT The cyclosporine is now established as the immunosuppressant of choice in human organ transplantation, because it improves graft survival. Unfortunately, the cyclosporine can lead to a wide spectrum of nephrotoxic complications. The aim of this study was to asses the effect of lacidipine (calcium antagonist) on the cyclosporine induced nephrotoxicity in rats. Thirty adult albino rats were used this study. They were divided into three groups: control group, cyclosporine group and lacidipine treated group. The rats of cyclosporine group were received a dose of 50 mg/kg of body weight for two weeks by daily oral administration. The rats of lacidipine treated group were received the same dose of cyclosporine for 14 days along with lacidipine lmg /kg of body weight by daily oral administration three days before and fourteen days concurrently with cyclosporine treatment. At the end of the experiment, there was marked elevation of serum urea and creatinine levels in the cyclosporine group. Lacidipine treatment significantly (P <0.05) reduced the elevated serum urea and creatinine. In control group, the histological examination of the rat renal cortex showed normal glomeruli and Bowman's capsule. The tubules which were seen in the sections consist mainly of the proximal convoluted tubules and some distal convoluted tubules. The proximal convoluted tubules are lined by columnar cells with rounded nuclei and prominent nucleoli. These cells have many microvilli which form a brush border. The cytoplasm of these cells contains mitochondria, few vesicle and small sized lysosomes. The renal corpuscles and tubules are surrounded by minimal amount of loose connective tissue. In cyclosporine group, the sections of the rat renal cortex showed necrosis of some glomeruli, thickening of the Bowman's capsule and degeneration of some tubules. There are hyaline degeneration of other tubules and marked deposition of condensed amount of connective tissue in the renal interstitium. The cytoplasm of cells lining the proximal tubules contains increased number of lysosomes, many vacuoles and vesicles. Some mitochondria are degenerated. In lacidipine treated group, the lacidipine treatment effectively prevented the cyclosporine induced changes in the renal cortex.