The potential protective effects of erythropoietin and estrogen on renal ischemia reperfusion injury in ovariectomized rats
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Background: Renal ischemia—reperfusion (RIR) is an important etiopathological mechanism of acute renal failure (ARF). Erythropoietin (EPO) has been candidate as a nephroprotectant agent. However, its nephroprotective effect when it is accompanied with estrogen has not been studied in female. Methods: Fifty-six female rats were divided into seven groups. Each formed of 8 rats. Group I: control group. Group 11: Female rats exposed to RIR (named RIR group).Group III: Female rats exposed to RIR and pretreated with EPO (named RIR + EPO group). Group IV: ovariectomized rats exposed to RIR (named OVR + RIR group). Group V: ovariectomized rats received estrogen (E) then exposed to RIR (named OVR + RIR + E group). Group VI: ovariectomized rats received EPO before RIR (named OVR + RIR + EPO group). Group VII: ovariectomized rats received E then received EPO before RIR (named OVR + RIR + E + EPO group).Serum creatinine, blood urea nitrogen (BUN) and renal blood flow (RBF) were measured. Tumor necrosis factor-a (TNE-ct). Myeloperoxidase activity (MPO), nitric oxide (NO), endothelin- I(ET-1) and EPO levels were assessed in the renal tissue. Histopathology was assessed to detect renal damage score. Results: RIR significantly increased the serum levels of creatinine and BUN with decrease in RBF. In addition it significantly increased TNF-a, MPO and endothelin-1 levels with decrease in NO and EPO levels in renal tissue. However, these parameters significantly reversed by EPO except RBF. Combination of E and EPO leads to significant decrease in the protective effect of FPO. Conclusion: It seems that EPO could protect the kidney against RIR, while this protective effect was decreased when E was supplemented. 0 2015 Alexandria University Faculty of Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (Imp: tleativecommons.orgilicenses,tby-ne-nd..4.0 ).