THE EFFECT OF SOME NON STEROIDAL ANTIINFLAMMATORY DRUGS ON THE ACTIVITY OF CARBONIC ANHYDRASE ENZYME IN EXPERIMENTAL RATS
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In this work, we study the effect of certain non steroidal antiinflammatory drugs (acetyl salicylic acid, indomethacin, naproxen, and piroxicam) on the activity of carbonic anhydrase enzyme in vitro and in red cell of experimental rats. We study the effect of these non steroidal anti-inflammatory drugs on the activity of carbonic anhydrase enzyme alone and in association with histamine, calcium, prostaglandin E2, and acetazolamide which is a specific inhibitor of carbonic anhydrase enzyme. The results show that, the non steroidal anti-inflammatory drugs used here increased the activity of carbonic anhydrase enzyme in a dose dependent manner. Histamine and calcium added to non steroidal anti-inflammatory drugs amplified the activating effect of non steroidal anti-inflammatory drugs on carbonic anhydrase enzyme. Association of prostaglandin E2 or acetazolamide (which is a specific inhibitor of carbonic anhydrase enzyme) to non steroidal anti-inflammatory drugs, reduced their activation on carbonic anhydrase enzyme. Indomethacin abolished the inhibitory effect of acetazolamide on carbonic anhydrase enzyme. We can say that the non steroidal anti-inflammatory drugs which reduce the activity of cyclooxygenase enzyme and prostaglandin production, increase the activity of carbonic anhydrase enzyme. These data imply that there is an inverse relationship between carbonic anhydrase enzyme and cyclooxygenase enzyme, that is to say carbonic anhydrase activation being accompanied by reduction of cyclooxygenase activity, a reduction may be achieved by the pH modifications induced by carbonic anhydrase activation. In this way, cyclooxygenase inhibition occurs via carbonic anhydrase with the pH variations it brings about.