EFFECTS OF CAFFEIC ACID ON HEPATIC ISCHEMIAREPERFUSION INJURY IN ALBINO RATS
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Background: During liver surgery and liver transplantation, ischemia-reperfusion (I/R) is an unavoidable and major unresolved problem. Therefore, various pharmacologic approaches to prevent hepatic UR injury are currently under trial. In this study, the effects of oral administration of caffeic acid on lipid peroxidation, antioxidant activity of superoxide dismutase and DT-diaphorase was investigated. Methods: Albino rats were classified into 3 groups. Group I served as sham operation, normal control group. In-group 2 and 3, the hepatic artery and portal vein were occluded for 30 minutes then reperfusion was done. Rats in-group I (normal control) and group 2 (non-treated I/R) groups received 1 ml saline orally. Rats ingroup 3 (treated I/R group), received caffeic acid (100 mg/kg) orally half an hour before occlusion. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured in serum of blood samples taken from cannulated carotid artery. Lipid peroxides content and superoxide dismutase (SOD) and DT-diaphorase activities were measured in liver homogenate 3 hours after reperfusion. Results: There was a significant elevation in the activities of AST and ALT in nontreated I/R group compared to sham operated control group, while in caffeic acid treated-UR group, there was significant reduction compared to non-treated UR group. Moreover, lipid peroxides in liver homogenate was significantly elevated in group 2 compared to group 1, while in caffeic acid treated group 3, lipid peroxides decreased significantly compared to group 2. SOD and DT-diaphorase in liver homogenate were significantly decreased in group 2 compared to group 1, while in caffeic acid treated group 3, there was significant increase compared to group 2. Conclusion: caffeic acid was proved to be protective agent against ischemiareperfusion induced hepatic injury in rats, an action that might be mediated through inhibition of antioxidant effect. This finding remains to be confirmed clinically.