TRAMADOL ENHANCES DOXORUBICIN — INDUCED NEPHROTOXICITY IN RATS
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Doxorubicin (DOX) is an anthracycline antibiotic which has a nephrotoxic effect. Tramadol(TRAM), an analgesic, which is often used to control acute and chronic pain. The aim of this study was designed to assess the nephrotoxicity effect of dazorubicin (DOX) and TRAM each alone and in combination. The rats were divided into four groups, control, DOX (4mg/kg, i.p), TRAM (54mg/lcg, i.p) and DOX&TRAM (4n2g/kg &54ing/kg,tp,respectively), doses were administered every three days for two weeks. At the end of the two weeks urine and blood were collected to evaluate serum creatinine, urea, creatinine clearance, urine volume. Also ratio of kidney weight to body weight (KW/B TV) was assessed. Kidney tissue were removed for determination of antioxidant enzymes ('reduced glutathione (GSH), glutathione S transferase (GST) and lipid peroxides (TBARS). Light microscopy was also done. The activity of reduced glutathione and GST were significantly decreased, level of TBARS was higher significantly in DOX and DOX & TRAM groups compared to control. Serum urea ,creatinine were increased significantly in DOX & TRAM group. AB W and FBIV were decreased significantly compared to control, also urinary flow rate was increased and creatinine clearance was decreased in DOX & TRAM group compared to control. On the other hand, light microscopic examination results showed that atrophy in glomeruli decreased in its cellularity in DOX treated group .also in sonic Bowman's capsule ,there was thickening in partial layer & narrowing in renal tubules. TRAM treated group showed normal tubules and atrophy in glomerulL DOX + TRAM treated group showed exaggeration in all features, vacculation and edema in glomeruli (fig 4 -7).