Epigallocatechin-3-gallate protects heart and kidney DNA from intestinal ischemia /reperfusion injury in rats
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ischemia/reperfusion (I/R) injury is considered as a major clinical problem It induced tissue injury and apoptosis mainly via oxygen free radicals,. Leading to the development of local and distant organ dysfunction, Objective: This study investigates DNA damage to the heart and kidney, as a result of intestinal UR, and possible DNA protection through the administration of epigallocatechin-3-gallate (EGCG). EGCG was chosen to be investigated in this model based on previous studies showed its antioxidants, anti- fibrotic and anti-apoptotic effects. Materials and methods: A total of 32 rats were randomly divided into four experimental groups: sham, UR, UR pretreated with EGCG (50 mg/kg ip 30 mm before ischemia) and EGCG treated (50 mg/kg ip). Apoptosis was assessed using comet assay. Indices of heart and kidney damage were measured (caspase- 3,8-hydroxydeoxyguanosine and heat-shock protein-70).Results: Intestinal UR caused heart and kidney damage as noted by significant increased DNA parameters (DNA tailed %, DNA untailed %, DNA Tail length , Tail DNA % and tailed moment), increased serum level of caspase-3,8- hydroxydeoxyguanosineand and heat-shock protein-70. EGCG significantly reduced DNA damage parameters on the comet assay in heart and kidney homogenate, and reduced serum levels of caspase-3,8- hydroxydeoxyguanosineand and heat-shock protein-70. Conclusion: Based on our results, we conclude that EGCG can protect the heart and kidney against intestinal UR injury via an anti-apoptotic mechanism.