Electron Microscopic Studies On Kidney And Liver Of Rat Following Therapeutic Doses Of Cefotaxime, Ofloxacin And Amikacin.
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The present work was performed to study the effects of commonly used three drugs ( cefotaxime, ofloxacin and amikacin) on some parameters of the liver and kidney functions of normal albino rats. In addition , liver and kidney of rats were examined by electron microscope for any histopathological changes. In this study, sixty male normal albino rats ( 120- 180 gm each ) were divided into 4 groups: 15 rats served as control, 15 rats treated daily with cefotaxime (30 mg / kg) , 15 rats treated daily with ofloxacin ( 10 mg / kg ) and 15 rats treated daily with amikacin ( 7.5 mg / kg ). Cetbtax i me and amikacin were given intraperitoneally (1.P) while ofloxacin was given orally in a freshly performed solutions 2 hours after feeding the animals. After 14 days from drugs administration, 10 rats from each group were used for examination. Blood samples were collected from the retrobulbar plexus and analysed for determination of total serum bilirubin, serum glutamic oxalacetic transferase ( SCOT), serunylutamic pyruvic transferase ( SGPT), serum alkaline pasphaye (ALP), serum urea and creatinine. After obtaining the blood samples, .the animals were killed and their livers and kidneys were removed and examined by the electron microscope for any histopathological changes. Five rats from each treated group were left without treatment for 7 days. After this period, all the above investigations were carried out. The results of this work as regards the liver functions showed that there was a singnificant increase in total serum bilirubin in both cefotaxime and ofloxacin treated goups, the increase induced by ofloxacin was more. In amikacin - treated group, liver functions were nearly toward normal. Concerning the renal functions, there was a singrtificant increase in serum urea and creannine in amikacm and ofloxacin - treated groups. All changes observed in the parameters measured were found to be transient and reversible as evidenced by return of the values to the control levels after stoppage of treatment. Histopathological results of liver and kidney showed no significant changes between any of the three groups which correlate with the reversibility of biochemical results. Therefore, it could be concluded that therapy with the above drugs must be controlled by regular assessment of the liver and renal function tests even in asymptomatic patients.