EFFECTS OF GAMMA RADIATION ON THE RAT LIVER AND THE PROTECTIVE EFFECT OF COMBINED VITAMIN E AND SELENIUM SUPPLEMENTS
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Abstract The biological effects of ionizing radiation result in the formation offree radicals which are the main cause of cellular damage. The aim of this study is to investigate whether vitamin E (as DL-a-tocopherol acetate) and selenium (as sodium selenate) exert a protective effect against radiation damage. Thirty adult male albino rats were used in this study. They were divided into three equal groups. The first group was served as control. The second group was given a fractionated dose of gamma radiation at a dose of I Gy, once weekly for eight weeks. The third group was given a daily intraperitoneal injection of DL-a-tocopherol acetate in a dose of 30mg / kg body weight and sodium selenate in a dose of 0.5 mg/kg body weight for two weeks before the start of radiation and during radiation. All experimental animals were then sacrificed and the livers were removed and prepared for both Light and electron microscopy. The liver cells of the irradiated group showed granular vacuolar cytoplasm with pylcnotic nuclei, moderate dilatation and hyperaemia of liver sinusoids, intense glycogen accumulation, an increase and gathering in the smooth endoplasmic reticulunt, swollen cisternae of the rough endoplasmic reticulurn, and a decrease in the cristae of the mitochondria that contain light matrix. In the irradiated treated group, the majority of liver cells returned normal, although some had still mild changes. Based on these morphological observations, it was concluded that the administration of DL-a-tocopherol acetate and sodium selenate exerts a protective effect against liver damage by radiation.