Publications of Faculty of Medicine:P-GLYCOPROTEIN EXPRESSION IN NEUROBLASTOMA: Abstract

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Multidrug resistance is a major obstacle in paediatric cancer Chemotherapy.P- glycoprotein is a transmembrane product of mdri gene that Acts as an efflux pump mechanism draining drugs out of the cells. Widely Disseminated neuroblastoma in children remains a disease of poor prognosis With high incidence of intrinsic and aquired drug resistance. The role of pglycoprotein And its prognostic value in neuroblstoma was investigated in a total Number of 32 cases, receiving either cyclofosfamide (ctx), platinol (cddp), vepesid (vp16), and adriamycin (adr) or vincristine (vcr), (adr), actinomyein-d (amd) and ctx. The mean age was 4.7 years and The male to female ratio was 1.The cases included 17 surgical specimens and 15 biopsies. Tumour grading was done according to shimada classification. Lmmunoperoxidase studies for p-glycopmtein were performed on formalin- Fixed, paraffin embedded sections using the isb-1 mouse monoclonal antibody, And light green as a counter stain.Cases were scored according to the Percent number of positive cells:strongly positive when more than 50% of Cells were stained,thildly positive when 11-50% were stained and negative When 0-10% of cells were stained.A high percentage reactivity for pglycoprotein Was detected in75% of cases, of which 58.3% were males. Positivity Was more in the unfavourable group according to the shimada classification (66.6%) and more with advanced stages 111 and iv (87.5%).A high Percentage of 83.3% of the p-glycoprotein positive cases died within the first Year of treatment. Conclusion: late clinical stages and unfavourable histology Correlate with high p-glyc.Protein expression and hence poor prognosis in Neuroblastoma