Publications of Faculty of Medicine:FIBRONECTIN IN SOME LIVER DISEASES: Abstract

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Urine and plasma samples from (100) patients with various liver diseases (hepatocellular carcinoma (HOC), liver cirrhosis, periportal fibrosis, acute viral hepatitis, fatty liver) and from (25) healthy individuals were collected and the proteins were separated by sodium Dodecy1 Sulphate Polyacrylamide Gel Electrophoresis (SDSPAGE), according to their molecular weights. These proteins were transferred on nitrocellulose paper and reacted against monoclonal anti-human fibronectin (FN) where a band of an apparent molecular weight of 155 Kilodalton (KDa) appeared. The results showed a sharp band in case of urine and plasma samples of patients with liver diseases at 155 KDa. There was no band in case of urine and plasma samples of healthy individuals. Also plasma FN was determined by ELISA technique, and showed a significant increase in the various liver diseases studied when compared to the control group (P<0.001). This may be due to increase in the number or the ability of the cells capable of fibronectin production and/or decreased consumption of fibronectin in various liver diseases. The sensitivity of Slott - Blot (SB - ELISA) for detection of fibronectin in urine is more than the sensitivity of ELISA for its detection in plasma. So fibronectin (FN) in urine can be used as a marker for liver diseases.