EFFECTS OF THYME EXTRACT ON HEPATOTOXICITY INDUCED BY FENITROTHION IN ADULT MALE MICE: A HISTOLOGICAL, HISTOCHEMICAL AND IMMMUNOHISTOCHEMICAL STUDY
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Fenitroth ion (Sumithion) is an organophosphorus pesticide, used worldwide in agricultural practice. his toxic to animals and humans. Thyme commonly used as food flavor and germ killer. The current work was undertaken with the objective to evaluate the protective effects of Thyme extract on hepatotoxicity induced by fenitrothion (sumithion) in adult male mice. Forty male mice were included in study and divided into 4 groups (each group included10 mice). Group I: the animals received tape water for 4 weeks. Group 11: The animals received daily oral dose offenitrothion" 1/30 LD50 ( 20 mg/kg body weight) by gastric tube for 4 week. Group 111: The animals received daily oral dose of Thyme extract (500mg/kg body weight/day) by gastric tube for 4 weeks dnd group IV) animals received bothfenitrothion and Thyme extract for 4 weeks. Liver specimens were preparedfor histological examination (H&E), histochemical examination of glycogen and protein and immuohistochemical detection of caspase-3. Finally quantitative analysis of DNA fragmentation was done. Microscopically group 11 showed many histological changes in liver sections in the form of loss of the hepatic architecture and apoptosis of hepatocytes with pre-cancerous changes that was supported by highly expressed caspase-3 reaction in the cytoplasm and nuclei of hepatocytes. Marked decrease PAS positive reaction and protein were detected in most hepatocytes. DNAfragmentation were significantly increased (32%) in comparison with all groups. Group IV showed improvement of histological and histochemical changes in the tissue. Thyme extract possess a hepato-protective against fenitrothion as it had antioxidant, anti-inflammatory and anti-genotoxic activities.