Apoptosis in Normal individuals, Rheumatoid arthritis and Osteoarthritis : Developing a new drug of natural origin
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The present study was designed to investigate the possible involvement of apoptosis of blood neutrophils in normal subjects, rheumatoid arthritis (RA) and osteoarthritis (OA) patients with and without some selected natural prouducts. This study conducted on 30 normal volunteers and 85 patients; 55 cases with RA ( active , and inactive) and 30 cases with OA of the knee . Blood neutrophils were prepared and cultured. The cells were incubated for 24,48 and 172 hours in tissue culture medium with and without some selected natural products (Ascorbic acid, Quercetin and Rutin).The percentage of neutrophil apoptosis in each culture was assessed by both morphology and DNA fragmentation.There was significent increase of the percentage of apoptotic cells with aging in culture mediun from normal subjects , RA and OA patientsin RA patients , there was inhibition of neutrophil apoptosis in comparison to normal subjects and OA patients in whom inflammatory response is less than in RA patients. There was no significant difference in the percentage of apoptotic cells between active and inactive RA patients. No relationship was found between apoptotic cell percentage and disease activity. Lastly, several observations have been made conseming the apoptotic inducing effect of selected natrual products.