COMPARATIVE STUDY BETWEEN ELISA IgG, IgM SEROLOGICAL TECHNIQUES AND PCR IN DIAGNOSING AND STUDYING TOXOPLASMOSIS IN QALUBYIA GOVERNORATE, EGYPT
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ELISA IgG, IgM antibodies and PCR for toxoplasmosis were done on 55 women with complicated gestation and their babies (abortion, premature. deliveries and deliveries of babies with congenital anomalies). ELISA IgM were done on 27 uncomplicated gestation (mothers & babies) in addition to 152 randomly selected individuals. Seropositivity to specific IgG antibodies was observed in 36.4%, 59.2% and 57.9% of complicated gestation, uncomplicated gestation and random population respectively. Only 27.3%, 7.4% and 10.5% were found seropositive for specc antitoxoplasma IgM respectively PCR was found positive in 20%, 50% and 60% of mothers with abortion premature deliveries and deliveries of babies with congenital anomalies respectively Also 55.5% and 40% were found seropositive for IgG from normal full term babies and abnormal babies respectively, only 13% of abnormal babies were IgM positive and 46.6% were PCR positive from the same group.