ULTRASTRUCTURAL CHANGES IN PANCREATIC ACINI AT DIFFERENT INTERVALS AFTER INDUCTION OF ACUTE HAEMORRHAGIC PANCREATITIS WITH DIAZINON
|Full paper||Not Available|
Thirty healthy adult male guinea pigs were utilized in this study. They were divided into six equal groups. The first was used as a control group. Acute partcreatitis was induced in the remaining 5 groups by a sublethal dose of the organophosphate insecticide Diazirton (DV. Animals of the 2nd, 3rd, 4th, 5th, and 6th groups were sacrificed 0.5,1,2,6 and 12 hours respectively after DZ ingestion. Pancreatic acinar alterations occurring after DZ intake can be arranged as follow: I) excessive stimulation of the pancreas appears after 0.5 hour, 2) initiation of intracellnlor autolytic changes and interstitial tissue degeneration after I hour, 3) appearance of peripheral vacuolation may be created by extracellular enzymes after 2 hours, 4) augmentation of peripheral vacuolation and occurrence of cellular necrosis after 6 hours. 5) disappearance of peripheral vacuoladon with reduction in the size of the acini after 12 hours. The interstitial tissues showed increasing width and degeneration. Conclusively, the contribution of intracellular activation of zymogen in the pathogenesis of DZ induced acute pancreatitis beside the other mechanism of increased intraductal hydrostatic pressure resulting from hypersecretion became evident Also, correlation between the ultrastructural changes in pancreatic acini and the time passing after DZ poisoning became manifest. This finding may pay the attention of medicolegal experts to put in their consideration the ultrastructure study of pancreas while investigating the obscure deaths.