Thirty semen specimens included in the study were taken from nonsmokers
and another thirty specimens from heavy smokers. Each
specimen was divided into two portions. One portion was centrifuged and
the pellet was resuspended in an equal volume of S.P. from the opposite
participant group (i.e. spermatozoa from a non-smoker into S.P. of a
smoker and vice versa). The other portion remained without processing.
Both portions were tested for sperm acrosin activity by gelatin substrate
film technique. Scoring the diameters of the haloes around sperm heads
did evaluation of the acrosin activity.
Before processing, the haloes of the nonsmokers were larger than
those of smokers indicating that their acrosin activity is higher. After
processing, the haloes around the heads of spermatozoa in nonsmoker
group specimens incubated in S.P. of smokers became smaller than before
processing indicating that their acrosin activity became lower. On the
other hand, haloes around the heads of spermatozoa in smoker group
specimens incubated in S.P. of nonsmokers became larger than before
processing. |
