Role of Helicobacter Pylori (H.pylori) Infection and Cyclooxygenase- 2 (COX-2) Expression in Chronic Gastritis and Gastric Carcinoma: An Immunohistochemical Study
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Abstract: Malignant tumors of the stomach are common, but the incidence of stomach cancer varies from country to another, probably as a result of genetic, epigenetic and environmental factors. Stomach cancer often occurs in older people whose stomachs produce only small quantities of acid. Although infection with Helicobacter pylori has been proven beyond doubt in the aetiopathogenesis of various gastric disorders, not much is known about the role of H.pylori infection in onset and progression of chronic gastritis as well as gastric cancer. Although recent studies have indicated that the clinicopathological parameters in patients with gastric carcinoma, the prognosis of advanced cancer still remains unsatisfactory. Objectives: This study aimed at investigating the expression of H pylori antibody and cyclooxygenase-2 (COX-2) in cases with chronic gastritis and gastric carcinomas and to correlate this expression with various clinicopathological parameters. Material and Methods: Paraffin sections from previously diagnosed chronic gastritis (CG) and gastric carcinomas (GC) were classified, graded and staged according to the updated for CO and the World Health Organization (WHO). for GC. Two sections were immunohistochemically stained for antibodies against H.pylori and COX-2. One section was stained with Feulgen stain for assessment of ploidy and proliferative activity using the Image analyzer system CAS 200. ConcluSion: Infection with H.pylori and cox-2 overexpression are common in patients with chronic gastritis and gastric carcinoma. Both anti-H pylori and cox-2 proteins are implicated in gastric carcinogenesis and their over expression may be a good predicator for worse prognosis and poor patient's outcome. Key Words: Helicobacter pylori — Cyclooxygenase — Stomach cancer—Gastric carcinoma — Immunohistochemical.