Nuclear Morphometry, Immortalization and Apoptosis in Laryngeal Cancer
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Background and Objectives: Laryngeal carcinoma is the most frequent neoplasm in the head and neck region and is notably prevalent in areas such as the Mediterranean basin. However, the numbers of reports that deal with the molecular biology of laryngeal carcinoma in this region are few. Thirtythree laryngeal cancer specimens and their adjacent mucosa were studied for the expression of telomerase catalytic unit (hTERT), bc1-X and bc1-2 together with morphometric analysis of nuclear parameters. Material and Methods: The standard peroxidase method was used For immunohistochemical localization of hTERT, bcl-X and bc1-2 antibodies where all antibodies and detection kits were purchased from Novocastra, UK. Feulgen staining was used before morphometric analysis. Results: Immunohistochemical detection of telomerase hTERT was found in 78.8% of cancers and in 75% of adjacent tissue and was comparable to previous results from other studies using TRAP assays. Low bc1-2 and high bcI-X expression was also detected in 18.2% and 60.8% respectively. A significant correlation could be found between high telomerase expression and tumor grade, nuclear area and nuclear short axis. Conclusion: Immunohistochemical detection of hTERT is comparable to other assays and could be related to higher tumor grade. Telomerase correlation with increased nuclear area and short axis, which are statistically correlated to malignant transformation could favor the theory of field cancerization in near-by mucosa.