Expression of C-erbB2 and CD44s is Association with Malignant Transformation and Progression in Cutaneous Squamous Cell Carcinoma
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Background: CD and C-erb-B2 isoforms have been reported to be involved in tumor invasion and metastasis in a variety of human cancers. Nevertheless, few studies have dealt with their expression in cutaneous squamous cell carcinoma (SCC). Objectives: To elucidate the role of C-erb-B2 and CD44s expression in cutaneous SCC in relation to its biologic behavior. Material and Methods: Paraffin blocks from 40 skin biopsies [23 SCC, 7 seborrheic keratosis (SK), 4 keratoacanthoma (KA), one squamous cell papilloma (SCP) and 5 normal skin controls] were cut and placed on poly-Llysine coated slides. The standard Streptavidin Biotin Peroxidase method was used after microwave antigen retrieval. Immunohistocheinical staining was evaluated semi-quantitatively and correlated with tumor type, degree of histological differentiation and invasion of resected surgical margins. Results: CD44s was expressed in normal skin, SK and SCC. High expression for CD44s (52, S3) was found in 17/23 (73.9%) of SCC cases and significantly correlated with that of C-erb-82 (p = 0.006), with advanced age (p = 0.031) and with safety margin invasion (0.026). C-erb- B2 was not expressed in normal skin, SK, or SCP but expressed in 19/23 (82.6%) of SCC and in 18/23(78-3%) of cases in epidermis overlying tumors. One case of KA (25%) was also positive for C-er-bB2 Conclusion: Absence of c-erb-82 in normal skin, SK and SCP contributes to its relation to malignant transformation in cutaneous SCC. Moreover, it could be used as a differentiating marker between keratoacanthoma and well-differentiated SCC. C044s overexpression could be related to aggressive behavior in this type of tumors.