Prognostic Influence of eosinophilic infiltrate, Cyciln DI and AgNORs in colorectal tumors
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The number of tumor associated eosinophil, Cyclin Di expression and argyrophilic nucleolar organizer region (AgNOR) and their correlation to clinicopathological factors in colorectal carcinoma were investigated. Material and methods: forty-four cases of colorectal carcinoma, 6 adenomenatous polypi free of cancer and 5 apparently nonnal mucosa (non-neoplastic, non-parasitic) as control were studied. All cases were formalin fixed, paraffin embedded and subjected to immunohistochemical staining fix Cyclin l)i using Al IC technique. Also the silver col bid technique was used fin determination of A gNOR. and (iiemsa stain to evaluate the eosinophil counts. Results: control and adammatous colonic polypi did not express Cyclin D1 while all types of colorectal carcinoma expressed it in different percentage, which could help in early detection of malignant transformation . Cyclin expression was significantly higher in Dukes, C colon cancer(p<0.05) and non-survivals (p410 I). Eosinophil counts was found to be low with poor histologic differrfiation and progression of tumors (p4).05). I Ugh eosinophil counts correlate significantly with survivors (p4).05). AgNOR score was significantly higher with increasing cell differentiation from control to adenomatous polypi to udenocareinomit groups and with 5-year survival (p<0.(I.5). I tut no significant correlation was found with Dukes' stage (p0.0.5). A significant inverse relationship could be found between eosinophil counts and Cyclin Di expression in all colorectal carcinoma cases examined (pc0.05). Conclusions: eosinophil counts and proliferative markers (Cyclin Di and AgNOR) could be valuable factors when combined for a beau prediction of colorectal carcinoma patient's outcome.