The protective role of viamin E and Angiotensin II receptor blocker on diabetic cardiomyopathy in male albino rats
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Diabetic cardiomyopathy is common complications of diabetes mellietus. There are many pathophysiological mechanisms of diabetic cardiomyopathy, such as apoptosis and oxidative stress. AIM: This study was designed to assess the issue of cardiomyocyte apoptosis as a possible cause of diabetic cardiomyopathy and whether it would be possible to suppress this apoptosis by the use of vitamin E as an antioxidant and Losartan as an Angiotensin II receptor blocker. METHODS: Rats were randomly divided into five groups 10 animals each: Group 1, healthy control rats; Group 2, diabetic group that were made diabeitic with a single injection of streptozotocin (Sl); Group 3, diabetic rats treated with losartan ; Group 4, diabetic rats treated with vitamin E Group 5, diabetic rats treated with losartan and vitamin E At the end of the experimental period, plasma glucose, serum lipid profile was measured. The heart rate and mean systemic arterial blood pressure (MSBP) were mea sured in all groups. Oxidative stress as assessed by malonaldialdehyde (MDA) and reduced glutathione (GSH-PX) concentrations as well as caspase-3 activity as an index of apoptosis were determined in cardiac tissue. In addition Cardiac apoptosis was measured by BCL-x immunohistochemisny technique.