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Title:
Protective Effect of Folic Acid on Prenatal & Postnatal Toxicity of Cisplatin on Spinal Cord '' A light and electron microscopic study ''
Authors: Saadia A. Shalaby, Essam M. Eid, Omar A. Aziz Allam, Naglaa A. S. Sarg and Abulmaaty M. Elsayed
Year: 2018
Keywords: cisplatin – spinal cord – folic acid.
Journal: Not Available
Volume: Not Available
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Local/International: International
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Abstract:

Background: cisplatin is used in chemotherapy of different cancers because of its apoptotic effects and its ability to generate Reactive Oxygen Species, but its usage during pregnancy is restricted because of its side effects including many malformations. As folic acid supplementation decreases the incidence of the neural defects caused by certain drugs as progesterone, this work investigates the possible protective effect of folic acid on cisplatin toxicity on spinal cord. Material & methods: I- (Prenatal group): Thirty pregnant rats were subdivided into three equal subgroups: A. prenatal control subgroup: received saline. B. Prenatal cisplatin subgroup: were injected intraperitoneally with the therapeutic dose of Cisplatin (1 mg /kg) on alternate days. C. Prenatal cisplatin & folic acid subgroup: They were injected intraperitoneally with Cisplatin Plus folic acid (400 microgram) by the nasogastric tube. The fetuses were obtained by caesarian section 2 days after the last dose to get the spinal cord.II- (Postnatal group): Thirty pregnant rats were subdivided into three equal subgroups A-Postnatal control subgroup: pups received saline from 1st day of delivery. B-Postnatal cisplatin subgroup: pups were injected intraperitoneally with the therapeutic dose of Cisplatin (1mg/gm.) on alternate days from 1st day of delivery. C-Postnatal cisplatin & folic acid subgroup: pups were injected intraperitoneally with the therapeutic dose of Cisplatin Plus folic acid in a dose of (400 microgram) by gastric tube. The specimens were prepared for light and electron microscopic examination. Results: Light microscopic observations: The spinal cord show signs of cisplatin toxicity in the form of degeneration & disfigurement of the grey matter with spots of hemorrhage. The neurons of the ventral horn have piknotic nuclei and surrounded by vacuolated white matter. The spinal cord of the folic acid groups show improvement which appear in the arrangement of the grey matter in dorsal and ventral horns. The central canal also become well defined and patent the neurons of the anterior horn have large nuclei and prominent nucleolus, thevacuoles in the white matter are less than that of the cisplatin group. Electron microscopic observations: the cisplatin affects the neurons which appear distorted with shrunken eccentric piknotic nuclei that consist mainly of heterochromatin. The nucleus is surrounded by irregular nuclear membrane. the cytoplasm of these neuron is full of vacuoles and dilated rough endoplasmic reticulum. The myelinated axons surrounding the neurons are vacuolated with destructed myelin sheath. The folic acid treated group show the neuron with signs of improvement which appear in the nucleus which consists of abundant euchromatin and less heterochromatin with regular nuclear membrane. The cytoplasm of these neurons contains multiple healthy mitochondria, rough endoplasmic reticulum and Golgi apparatus with no vacuoles; the myelinated axons are surrounded by intact myelin sheath. Conclusion: cisplatin has a potent toxic effect on the developing spinal cord which may extend to the degeneration of the spinal cord. Folic acid can alter this toxicity and protect the developing spinal cord. [Saadia A. Shalaby, Essam M. Eid, Omar A. Aziz Allam, Naglaa A. S. Sarg and Abulmaaty M. Elsayed. Protective Effect Of Folic Acid On Prenatal & Postnatal Toxicity Of Cisplatin On Spinal Cord '' A light and electron microscopic study ''. Nat Sci 2018;16(4):96-104]. ISSN 1545-0740 (print); ISSN 2375-7167 (online). http://www.sciencepub.net/nature. 16. doi:10.7537/marsnsj160418.16.

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