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Assist. Amany Mohamed Emam :: Publications:

Title:
Investigation of fungus associated within cooccurrence of aflatoxins and ochratoxin a in cereals from Egypt
Authors: El Sayed TI,1 El Desouky TA,2 Abd El Aziz AM
Year: 2019
Keywords: aflatoxins, ochratoxin A, occurrence, fungi and cereals MOJ
Journal: MOJ Toxicol
Volume: 5
Issue: 3
Pages: 92‒99
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Amany Mohamed Emam_Investigation of fungus associated within cooccurrence of aflatoxins and ochratoxin a in cereals from Egypt.pdf
Supplementary materials Not Available
Abstract:

The present study was prepared to investigate fungi associated within Co-occurrence of aflatoxins (AFs) andochratoxin A (OTA) in some cereals using High Performance Liquid Chromatography (HPLC) to compare the levels of contamination with the Egyptian standard (ES) and European unions (EU) regulation. Sixty cereals samples (white corn, yellow corn, wheat and polished rice) were collected from different localities in Qalubyia governorate. The results indicated that Total Fungal count (TFC) isolated were 535 isolates in non-sterilized cereals and 276 isolated with sterilized cereals. Also data showed that the most isolated fungal species were identified as. Aspergillus, Penicillium, Fusarium, Rhizopus, Alternaria and Cephalosporium Allergy. It was observed that Aspergillus spp. (A. flavus, A. niger and A. parasiticus) were the most frequent in all samples. When testing the ability of isolated fungi especially Aspergillus flavus, Aspergillus parasitic us to produce for AFs as well as Aspergillus niger and Aspergillus ochraceous for OTA. Results clarified 24 isolates of A. flavus and10 isolates of Aspergillus parasitic us were producers for AFs. On the other hand the obtained results indicated that 17 and 3isolates of Aspergillus niger and Aspergillus ochraceous were producers for OTA, respectively. The result indicated that the percentages of the incidence of AFs in samples were 60%, 46.6%, 26.6% and 33.3% with white corn, yellow corn, wheat and rice, respectively. Accordingly to results six samples exceeded the maximum levels of AFs set in the EU and ES. While all the rice samples under study were within the permissible limits as recommended by the ES and EU. On the other hand the incidence of OTA in cereals samples are 40%, 26.6%, 20%and 20% of cereals samples (yellow corn, wheat, rice and white corn), respectively. According to obtained results OTA in samples were less than the regulatory limits as recommended by ES and EU.

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