Impact of Using Brilliant Cresyl Blue Stain on Oocyte and Embryo Selection [2018-01-02]
T HE SUCCESS of in vitro embryo production is ultimately dependent on the number and quality of cumulus-oocyte complexes harvested from ovaries. The morphological criteria are routinely used for oocytes selection in most laboratories. There is brilliant cresyl blue stain (BCB) test allowing the selection of developmentally competent oocytes which is a simple, quick, economic and feasible protocol. BCB is known to be a non-invasive methodology that allows the selection of immature oocytes in several species. BCB test determine the intra cellular activity of glucose-6-phosphate dehydrogenase (G6PDH) that gradually decreases its activity as oocytes reach their growth phase. The using of BCB test before IVM is still controversial, so this review describes the use of BCB staining test for oocytes and embryo selection to investigate the impact of application of this stain in in-vitro maturation and embryo production in animals.
Key words: Oocytes quality, brilliant cresyl blue stain, embryo quality, selection, in vitro maturation.
Factors Affecting Buffalo Oocytes Maturation [2018-01-02]
Abstract: in vitro maturation, fertilization and culturing of buffalo oocytes were considered the most important
application, to improve buffalo reproductivety and productivity. Oocyte maturation is the first and most critical
step towards successful in vitro embryo production. There are many factors affect the maturation of buffalo
oocytes as culture media, method of recovery, oocyte quality, season of collection and ovarian status and
others. Proper oocytes selection in the laboratory is crucial for successful embryo production in vitro. Little
information is available on in vitro maturation and fertilization of buffalo oocytes. in vitro fertilization results
obtained by several workers were relatively poor when compared to cattle. So the present review aimed to throw
some lights on the factors affecting oocytes in vitro maturation in buffalo.
Key words: in vitro Maturation Buffalo Oocytes
Effect of Season of the year and Ovarian Structures on Oocytes Recovery Rate, Quality and Meiotic Competence in Egyptian Buffaloes Al-shimaa Al-H. H. El-1 Naby, 2Karima G.H.M. Mahmoud, 2Youssef F. Ahmed, 1Mahmoud E.A. Abouel-Roos and 1Alaa E. Abdel-Ghaffar [2018-01-02]
Abstract: The present study aimed to evaluate the effect of season of the year and ovarian structure
(corpus luteum) on the recovery rate, quality and maturation of Egyptian buffalo oocytes. For this purpose,
ovaries were collected from slaughtered buffaloes and oocytes were scored after recovery by aspiration.
Oocytes were matured in vitro for 24 h and evaluated for nuclear maturation after staining by 1% orcein stain.
Results showed a significantly (P