PASTEURELLA MULTOCIDAIN CAMELS: INCIDENCE,CAPSULAR AND
VIRULENCE GENES CHARACTERIZATION
1
Ashraf A.Abd El Tawab,
1
Fatma I.El -Hofy,
2
Attia Al-Jeddawy and
2
Ebtehal Abo-Hamdah
1
Bacteriology, Immunologyand Mycology Department,Faculty of Veterinary Medicine Benha
University .
2
Animal Health Research Institute, Dokki – Giza.
ABSTRACT
Pasteurella multocida is the main cause of haemorrhagicsepticaemia in camels.This
study looks at the isolation and molecular examination of haemorrhagicsepticaemia in
camels from May 2014 to March 2016 in30 nasal swabs from MarsaMatruh and 120
lungs (70 lungs from camels slaughtered in Basateen abattoir in Giza Governorate
and 50 from camels slaughtered in Al-Shohada abattoir at Al-Menofia Governorate)
which were collected and subjected to clinical and postmortem examination as well as
for bacteriological and molecular examination.Pasteurellamultocida was isolated
from total samples at an incidance of 5(3.3% ).While the percentage of the isolation
rate from 120 camel lungs was 5(4.2%) and from 30 nasal swabs was( 0%).In the
pathogenicity test all P.multocida isolates were highly pathogenic . P.multocida
isolates were identified by mPCR and23SR NA gene was amplified at 1432bp .Three
out of total five isolates were identified as P.multocida type B with amplification at
760bp while the rest 2 isolates identified as P.multocida type A and amplified at
1044bp . Also PCR showdthat toxA gene was amplified in all isolates and giving
product of 864bp but ptfA gene was not detected.It was concluded from this study
thatP.multocida in camels can be diagnosed with different methods such as
confirmatory biochemical and molecular assays |
