Rift Valley Fever (RVF) caused by an arbo-virus belonged to genus Phlebovirus, family Bunyaviridae, is an acute, febrile disease of ruminants. In Egypt, immunization of susceptible animals occurs using a locally prepared inactivated vaccine with aluminum hydroxide gel adjuvant. Adjuvants play an important role in vaccine formulation, so, selection of the proper adjuvant can elaborate high and long standing immunity. The aim was to develop a new RVF vaccine with paraffin oil adjuvant in water in oil in water (W/O/W) formula using Tween80 and Arlacel A as surfactants. Evaluation of the immune response to the prepared RVF vaccine in sheep was done in comparison with the local RVF vaccine with using SNT and ELISA. Results gave the priority to the prepared inactivated RVF vaccine with Paraffin oil adjuvant that induced high immunological enhancement without toxicity and with longer duration of immunity that extended for 9 months. |
