Numerous of Cases of skin nodules eruptions tied to lumpy skin disease virus (LSDV) are becoming more frequent in Qailubia Province, Egypt amongst cattle populations in 2014. In this study, a strain of LSDV (LSDV-Elkady-2014) associated with that outbreak was isolated from scabs of clinically infected Frisian cattle on chorioallantoic membrane (CAM) of specific pathogen free embryonated chicken eggs (SPF-ECEs). The isolate induced hemorrhage, enlarged liver, slight heart hypertrophy of chick embryos, with a pock lesion on CAM. Identification of LSDV-Elkady-2014 isolate was confirmed by histopathology, electron microscopy (EM), immunoflourescence (IF), and polymerase chain reaction (PCR) targeting LSDV envelope protein-like gene. Based on the partial sequence analysis of LSDV envelope protein-like gene. LSDV strain was phylogenetically related to LSDV- NI-2490, LSDV/ NW/LW, and LSDV/2/slemani/Kurdistan/ 2014. In conclusion, LSDV is still circulated in Egypt, and the isolated strain induced characteristic properties on chick embryos that were not previously recorded by LSDV. |
