Abstract: Background: Exposure to carbon tetrachloride (CCl4) initiates nephrotoxicity in rats. The purpose of the
present work was to study the role of bone marrow- derived mesenchymal cells (BM-MSCs) on CCl4-induced
nephrotoxicity in adult male albino rat judged by light and electron microscopeusing histological,
immunohistochemistry and ultrastructure parameters. Material & methods: Thirty adult male albino rats were
classified randomly into four experimental groups. Group I: Control group which was equally divided into two
subgroups; Ia (thatwas fed on the standard diet) and Ib (that injected intraperitoneally with 0.1 ml of olive oil twice
weekly for 4 weeks ). Group II: treated with 1.0ml /kg b.w of 10% CCl4 dissolved in olive oil, IP, twice a week for 4
one month. Group III: was injected intraperitonealy with the same dose of CCl4 twice a week for 4 weeks and then
were given a single dose intraperitoneally of 3×106 BM-MSCs. Group IV: served as recovery group, were treated IP
with 5.0ml /kg b.w of 10% CCl4 twice a week for 4 weeks and left for a further 4 weeks. At the end of experimental
period, all of the rats were scarified and both kidneys were prepared for histological, immunohistochemical and
ultrastructural study. Results: In CCl4treated animals, there were several pathological changes included: dilatation
of tubular lumens with intraluminal cell debris and vacuolation of tubular epitheliumas compared with the control
rats. Mallory’s trichrome staining showed increased amount of collagen fibers in the glomerulus and interstitium in
CCl4 treated animals. While the rest of groups showed minimal collagen fibers around glomerulus and renal tubules.
Immunohistochemical staining using α-Smooth muscle actin (α-SMA) showed negative reaction in all groups except
CCl4 treated animals and recovery group. EM evaluation of CCL4 induced changed revealed significant
condensation of chromatin in the nucleus and shortened brush border of proximal convoluted tubules, swollen
mitochondria and loss of apical plasma membrane micro projection of distal convoluted tubules, also effacement of
secondary foot processes on basement membrane of glomerular capillary with loss of slit diaphragms. In contrast,
these deleterious histopathological alterations resulting from CCl4nephrotoxin were absent after BM-MSCs
treatment in CCl4 + BM-MSCs group of rats. Conclusion: Our results demonstrated the protective potential of BMMSCs
on CCl4-induced nephrotoxicity in adult male albino rat with better recovery findings in BM-MSCs received
group than in those rats that were left to recover without treatment. |
