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Dr. Enas Abdelbaset El-sayed Soliman :: Publications: |
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| Title: | A Rapid molecular method for identification and differentiation between T.equinum species isolated from dermatophytic horses. |
| Authors: | Ali M. El-Ged, Adel M. Khalid, Ashraf A. Abd El-Tawab and Enas Abd El-Baset. |
| Year: | 2011 |
| Keywords: | dermatophytes, T.equinum, PCR |
| Journal: | Benha Veterinary medicicine J |
| Volume: | Not Available |
| Issue: | Not Available |
| Pages: | Not Available |
| Publisher: | Not Available |
| Local/International: | International |
| Paper Link: | Not Available |
| Full paper | Inas Abdelbaset El-sayed Soliman_Research 1.pdf |
| Supplementary materials | Not Available |
| Abstract: |
T. equinum is the main cause of dermatophytosis in horses, all over the world the identification and differentiation between T.equinum species by conventional methods has drawbacks of being very slow, often requiring weeks of culture and suffering complications of overgrowth with commensals so the molecular approaches are applied recently for diagnosis of dermatophytes. This study involved identification of T.equinum by conventional methods and application of PCR by using non-specific simple repeat sequence (GACA)4. The result showed that identification of T.equinum var. equinum and T.equinum var. autotrophicum and the primer was able to amplify both species forming characteristic PCR profiles for each. |
