Background: Rebamipide is a cytoprotective agent that enhance epithelial cell regeneration, with antiinflammatory and antioxidative properties. Mesenchymal stem cells derived-microvesicles have antiinflammatory, immunomodulatory and regenerative effects on damaged tissue. So, Rebamipide and microvesicles can be used for promotion of tissue regeneration.
Aim: The aim of this study is to evaluate the possible beneficial effect of rebamipide and mesenchymal stem cells derived microvesicles on treatment of induced ulcerative colitis.
Material and methods: 50 adult male albino rats were divided into 5 equal groups. Group I (control group) Rats injected saline intra-rectal. Group II (Colitis group) Rats injected 1 ml/rat/day of 5% acetic acid for 1 week. Group III (Rebamipide group) rebamipide will be prepared as a suspension in 1.5% carboxy methyl cellulose in water and administered rectally in a dose 1 mL/kg 24 hours after induction of colitis, for 14 days. Group IV (Microvesicles group) each rat received a single dose of 15 μg of MVs dissolved in 0.5 ml of phosphate buffered solution (PBS) delivered intravenously. Group V (microvesicles and rebamipide group) after induction of ulceration, they received microvesicles and Rebamipide as befor. The colon samples will be taken for, (1) Histological Method: H&E staining & alcian blue stain (2) Immunohistochemical methods: TNF alpha for detection of cytoplasmic inflammation and PCNA for detection of nuclear regeneration.
Results: Control group showed normal structure of colonic mucosa with surface columnar absorptive cells and goblet cells, lamina propria with regular arranged crypts, normal mucularis mucosa and submucosa. Group II showed ulceration and crypt abscess. The Lamina propria and submucosa show heavy inflammatory cell infiltration and congested blood vessels. Group III showed apparently normal mucosa with discontinuity in certain areas. Mild inflammatory cell infiltration and congested blood vessels in lamina propria and submucosa. Group IV showed intact mucosa with continuous simple columnar absorptive cells with goblet cells. Lamina propria showed nearly regular arranged crypts and few inflammatory cell infiltration. Group V showed intact mucosa with surface columnar absorptive cells and goblet cells. Regularly arranged crypts with connective tissue in between. Sub mucosa showed normal structure.
Conclusion: rebamipide and microvesicles provide a therapeutic strategy for managing induced ulcerative colitis by acetic acid in rats.