High level of somaclonal variation is a serious problem appears during stages of plant tissue culture. This phenomenon stands as a huge barrier in face of utilizing genetic transformation methods on a commercial scale. Somaclonal variation strongly related with long regeneration periods. At the same time main reason to prolong regeneration period is the long intermediate callus phase which increases and also promotes dedifferentiation leading to somaclonal variants. In this study two economically important, tetraploid potato cultivars designated Albatros, and Desiree were transformed relatively in a short period of time. A simple and rapid transformation protocol was used. After only one week on callus induction media Green, friable and nodular callus was formed. Callus induction frequency of the two genotypes Albatros, and Desiree was 59.7%, and 68.6% respectively. Average of shoot bud formation of the two genotypes Albatros, and Desiree was 46.7% and 39.1%, respectively. While, average regeneration of shoot bud was 27.9, and 25.6 for the two genotypes Albatros, and Desiree, respectively. Regenerated shoots were transferred to rooting media supplemented with 80 mg/l kanamycin. Overall, transformation efficiency of the two genotypes Albatros, and Desiree was 13.5%, and 9.1%, respectively. Complete regenerated plants were transferred to micro-tuberization media. 13 plants succeeded to form micro-tubers within ten weeks. The resulted plants did not show any significant morphological changes compared with control plants. |
