Medicinal and aromatic plants production has been rapidly increased in recent years and has played an important role in the national income, this study was conducted during two seasons (2010/201 1 and 2011/2012) to investigate the effect of sorne microelernents ( Iron, manganese the zinc each at 13% EDTA) and various sources of bio-fertilizers (Nitrobein, phosphorein and potassiurnage) and their interacرions on growth, volatile oil contents and chemical composition of rosemary (Rosmarinus officinalis L. plants.
The microelements treatments were; control (without any addition) and Fe at” 50,100 and 150 ppm” Mn and Zn each at “75, 100 and 125 ppm”. Three kinds of bio-fertilizers Nitrobein 1 g/rn2, phosphorein 1 g/m2 and potassiurnage 1 g/rn2, phosphorein 1 g/rn2 and potassiurnage 1 g/rn2, phosphorein 1 g/rn2 and potassiurnage lg/rn2. The interactions treatments were (Fe + Mn + Zn each at lOOpprn + Nitrobein 1 g/rn2), (Fe + Mn + Zn each at 1 OOppm+ phosphorein 1 g/rn2), (Fe+ Mn + Zn each at lOOpprn + potassiurnage 1 g/ rn2) and (Fe + Mn + Zn each at lOOpprn + Nitrobein + phosphorein + potassiurnage each at lg/rn2) on growth volatile oil contents and chemical composition of rosemary plants. The results emphasized that spraying with rosemary plants Zn at 100 ppm produced the tallest plants, the greatest number of branches plant, the highest content of carbohydrates, N, P, K, Zn and essential oil in the herb otherwise praying of Mn at 75 ppm produced the heaviest fresh and dry weight of herb. Moreover Mn at iOOpprn was increased total leaf content of chlorophyll a and b and carotenoids . Bio-fertilizer Nitrobein was increased all studied parameters of plant height, number of branches! plant fresh and dry weights per plant and per feddan. Also, the best results of chlorophyll a, b and carotenoids, total carbohydrates, N, P, K, Mn and Zn contents in the leaves and total percentages of essential oil were gained by application . • 2 ‘ • of Nitrobem 1 g/rn on compared with phospherew, potassiumage and control treatments. The combination treatment between microelernents (Fe+ Mn+ Zn each at 1 OOppm) + bio- fertilizers (Nitrobein + Phosphorein + Potassiumage each at lg/m2) was the most preferable treatment among other all studied treatments. The superior interaction treatment maximized plant height, number of branches plant, fresh and dry weights per plant and per feddan, in addition the above mentioned interaction treatment showed superiority in all of chemical traits and increased entages and the main component of essential oil such as a-pinene, Camphene, 13—Pinene, Lirnonene, Cineol Linalool, Camphor, a—Terpineol, Borneol, Bornyl acetate, Eugenol and 3-Caryophyllene.