|You are in:Home/Publications/Role of cytogenetic studies, protein electrophoresis and ISSR markers in assessment of genetic diversity inStevia rebaudiana (Bertoni).|
Prof. Makhlouf Mohamed Mahmoud Bekhit :: Publications:
Role of cytogenetic studies, protein electrophoresis and ISSR markers in assessment of genetic diversity inStevia rebaudiana (Bertoni).
|Authors:||Elham Shehab, M. M. M. Bekhit, M. S. Abdel-Sabour, T. A. M. El-aqaad and and A. A. El-Sheikh|
|Keywords:||chromosomes, Stevia, bivalents, SDS-PAG Eelectrophoresis, ISSR|
|Journal:||4th International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, Moshtohor and Hurghada , 4-7 April 2018 , Egypt|
|Publisher:||Faculty of Agriculture, Moshtohor|
|Paper Link:||Not Available|
|Full paper||Makhlouf Mohamed Mahmoud Bekhit_Final 16 PB Elham Shehab ICBAA 2018.pdf|
|Supplementary materials||Not Available|
Chromosome number was investigated in a number of strains of Stevia rebaudiana. The chromosomes were analyzed during mitosis and different stages of meiosis. All stevia strains were found to had 2n = 22. The number of bivalents at diakinesis was 11 for all the studied strains. Analysis of SDS-PAGE of soluble protein revealed complete similarity (100%) among the six strains of Stevia while two strains (broad and narrow leaves) are different in one band. Eight genotypes were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships. The level of polymorphism detected among the eight strains of stevia was 100.00% using inter simple sequence repeat (ISSR). A total of 231 clear bands were generated, out of which 34 (14.72 %) were unique bands. The total number of markers varied from 1 to 12 with a mean of 5.67 markers per primer.