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Prof. Makhlouf Mohamed Mahmoud Bekhit :: Publications:

Cytological and Molecular studies in some interspecific hybrids resulted from crossing between tetraploid and hexaploid wheat
Authors: Seham M. Demais, M. M. M. Bekhit, M. S. Abd El-Sabour, T. M. S.Salem and K. I. Gad
Year: 2018
Keywords: wide hybridization, Triticum, SDS-PAGE, Fraibilin, Laggards, meiotic anomalies, tetraploid wheat, bread wheat.
Journal: 4rd International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, Moshtohor and Hurghada , 4-7 April 2018 , Egypt
Volume: 1
Issue: 1
Pages: 383-400
Publisher: Faculty of Agriculture, Moshtohor
Local/International: International
Paper Link: Not Available
Full paper Not Available
Supplementary materials Not Available

Wheat relatives are rich sources of beneficial genes that are capable to change the situation of the wheat strains. The present study attempted to cross three tetraploid wheat strains as male parents with five strains of hexaploid bread wheat to understand the behavior of hybrids in relation to cytology, Scanning Electron Microscopy for wheat grains and SDS-PAGE (Friabilin). Scanning electron microscopy of dry grains revealed morphological details of grain surface structure. The grains of strain 20 cultivar showed narrower size and hairless area than the grains of strain 9 or 17 or 6. The hairy end of the strain 17 grains is broader than the same end (hairy) of the strain 21. The hairy end of the strain 24 is completely disappeared from grains. All the produced six crosses were containing hairy ends as an indicator for the inheritance of this character. Probably complete dominance of the gene controlling the brush area in the wheat grain. Various degrees of chromosome anomalies were seen with all the hybrids between T. durum and T. aestivum. D genome from T. aestivum could enhance more lagging chromosomes rather than genomes A and B of cultivated species. The main observed abnormalities in the produced crosses are the laggard formation. Fraibilin clearly associated with genetic proximity of the species, which characterized themselves by maintaining the genome homeology.

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