The present study aimed to early investigation of hepatocarcinogenic effect of poly vinyl chloride
on male albino rats in order to improve cancer therapy through studying the toxic effect of PVC on some
biochemical changes as ALT, AST, ALP and alfa-fetoprotein as tumor marker. Also study the changes in
m-RNA expression of cytochrome P450 2E1 (CYP2E1) and tumor suppressor gene (TP53) as early cancer
biomarker. Sixty male albino rats used in this study and classified to 3 groups: Group I used as control, while
Group II (1/20 LD of PVC) and Group III (1/10 LD of PVC) were given PVC orally v 50 50 ia stomach tube three times
per week for 2 months. The results were more pronounced on increase of some biochemical parameter
(ALT, AST, ALP and alfa fetoprotein) and m-RNA expression of tumor suppressor gene (TP 53) at the first and
second month of the experiment. Also found an increase of m-RNA expression of cytochrome2E1 gene
(CYP2E1) at first month and decrease at second month. This study provided strong evidence that application
of gene expression profiling to preneoplastic liver diseases and hepatocellular carcinoma is particularly
important in early investigation of carcinogenic risk by toxic agent like PVC.
Key words: Poly Vinyl Chloride Hepatocellular Carcinoma Gene Expression Alfa Fetoprotein |
