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Dr. Prof. Mohamed Mahmoud Moustafa Kandiel :: Publications:

Effect of honey supplementation on Egyptian buffalo semen
Authors: Mohamed Mahmoud Moustafa kandiel, Ahmed Reda Mohamed Elkhawagah
Year: 2017
Keywords: buffalo, chilled semen, frozen semen, honey, in vitro fertilization, skimmed milk, spermatozoa
Journal: Anim. Reprod
Volume: 14
Issue: 4
Pages: 1103-1109
Publisher: Not Available
Local/International: International
Paper Link:
Full paper Mohamed Mahmoud Moustafa Kandiel_p1103-1109 (AR891) Final paper.pdf
Supplementary materials Not Available

This study testified the effect of honey supplementation (0.5-4.0%) in milk on the quality of chilled and frozen buffalo spermatozoa. Semen was chilled with/without honey and examined for motility, viability, plasma membranes integrity by hypo-osmotic swelling test (HOS) at 0, 1, 2 and 4 h. Frozen-thawed semen was examined for the same criteria beside the viability index and in vitro cleavage rate. The motility, livability and HOS of chilled semen upsurge with honey supplementation 1.0-2.0%. The normality of chilled spermatozoa was improved in the presence of 2.0-4.0% of honey at 4 h. Tail abnormalities decreased with milk-honey 0.5, 1.0 and 2.0% at 2, 1 and 4 h, respectively. Incorporation of honey in milk extenders at levels of 0.5-2.0% was associated with an enhanced post-equilibration motility. The post-thawing motility showed a steady increase with honey levels. The viability index increased (P < 0.001) with milk-honey 2.0% (109.00 ± 9.91) and 4.0% (112.00 ± 14.41). In vitro cleavage rate was clearly (P 0.001) enhanced in the co-existence of milk-honey 2.0% compared with control (74.00 vs. 45.83). In the meantime, a reasonable high cleavage rate (67.00%) was encountered with milk-honey 0.5%. In conclusion, incorporation of honey in skim milk extenders is promising to enhance the characteristics and fertilizing potential of stored buffalo’s semen due to its nutritive and protective properties

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