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Dr. Mohamed El-Raey Mohamed Metwally :: Publications:

Title:
EFFECT OF MELATONIN ON BUFFALO BULL SPERM FREEZABILITY, ULTRASTRUCTURE CHANGES AND FERTILIZING POTENTIALS
Authors: MOHAMED EL-RAEY; BADR, M.R.; ASSI, M. M and RAWASH, Z.M.
Year: 2015
Keywords: Melatonin, semen extender, cryopreservation, HOST, ultrastructure and conception rate.
Journal: Assiut Vet. Med. J.
Volume: 61
Issue: 144
Pages: 201-208
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Not Available
Supplementary materials Not Available
Abstract:

Cryopreservation induces sublethal damage to the spermatozoa, which leads to reduce their fertile life. The objective of the present study was to investigate the effect of melatonin on freezability, ultrastructure and fertilizing potentials of the buffalo spermatozoa. Buffalo spermatozoa were cryopresreved with Tris egg yolk extender containing 7% glycerol supplemented with (0.100, 0.250, 0.500, 0.750 and 1 mM melatonin) or Tris-based extender only (control). Cryopresreved spermatozoa were assessed for post-thawing sperm motility, viability and acrosomal integrity, ultrastructure changes and fertilizing potentials. The current results clearly indicated that adding 0.1 mM melatonin to Tris extender significantly improved (P

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