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Dr. Mohamed Salah Omar :: Publications:

Title:
Antileukemic Activity of Glycated Fibrinogen-Methotrexate Conjugates
Authors: URSZULA KANSKA, MOHAMED SALAH OMAR, RENATA BUDZYNSKA, DMITRY NEVOZHAY, MONIKA JAGIELLO, ADAM OPOLSKI and JANUSZ BORATYNSKI
Year: 2005
Keywords: Fibrinogen, methotrexate, conjugates, glycation, antitumor activity.
Journal: ANTICANCER RESEARCH
Volume: 25
Issue: Not Available
Pages: 2229-2234
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Mohamed Salah Omar _Antileukemic Activity 2005.pdf
Supplementary materials Not Available
Abstract:

Background: The aim of the study was to compare the antileukemic activity of methotrexate (MTX) conjugates with native and glycated fibrinogen. We expected that conjugates based on glycated fibrinogen would reveal higher antileukemic activity because of decreased plasmin digestibility and a higher retention rate of glycated fibrinogen in the body. Materials and Methods: Fibrinogen was glycated using a high-temperature procedure at 65-85ÆC. Glycated fibrinogens were examined with respect to their ability to clot and susceptibility to plasmin digestion. Native fibrinogen (F) and fibrinogens glycated at 65 and 73ÆC (F65 and F73) were conjugated with MTX and tested in mice bearing P388 leukemia, at a dose of 40 mg of MTX per kg of body weight. Results: Glycated fibrinogens retained their ability to clot. Compared to native fibrinogen, they were more resistant to digestion by plasmin. All tested conjugates revealed higher antitumor activity than the free drug. Increases in average lifespan over the control group were 34% for free MTX, 137% for F-MTX, 151% for F65-MTX and 91% for F73-MTX. The differences between the antitumor activities of all conjugates were not statistically significant. Conclusion: It seems necessary to compare the antitumor activities of MTX conjugates based on native and glycated fibrinogen in different tumor models, to demonstrate the expected differences.

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