Significance of p27,p53,BAX, and CD44 expression in papillary thyroid carcinoma in predicting for cervical lymph node metastasis
Ragaey R. Fahmy MD
Department of pathology, Benha faculty of medicine
a b s t r a c t
Expression of p27,p35, Bax and CD44 was investigated in 30 papillary thyroid carcinoma (PTC) with or without cervical nodal metastasis to determine the role of immunohistochemical marker for prediction of neck metastasis in these patients.
P27 expression in non-metastasizing PTC was lower than normal thyroid tissue (P≤ 0.01) and higher than metastasizing PTC (P≤ 0.01), p53 immunoreactivity was present, faint (grade 1) in 2 cases (11%) and moderate (grade 2) in one case ( 5.5%) of non-metastasizing PTC, while 15 (83.5%) cases on non-metastasizing PTCs and all of metastasizing PTCs and normal thyroid tissue had no immunoreactivity for p53. There was no statistically significanct difference among all groups of immunoreactivity for p53. Expression of Bax in patients with PTC was more than normal thyroid tissue ( P≤ 0.01). However, non-metastasizing and metastasizing PTC was similar. The results indicate that the metastasizing PTC showed significantly low CD44 expression than the non-metastasizing PTC (p ≤ 0.05), also the metastasizing and non-metastasizing PTC showed significantly less CD44 expression than the normal tissue (p≤ 0.05).
The immunohistochemical evaluation of p27 and CD44 expression in patients with PTC may be useful to predict for metastasis risk of the PTC to regional lymph nodes.
Research in Benha M.J. 24 (3)(2007) 593–609