Urinary tract infection is the second most common type of
infection in the body caused mainly by E.coli which is responsible for
75-90% f uncomplicated cystitis in younger women.(Foster et al.,2008).
Acquired antimicrobial resistance is a growing worldwide problem due to
the increasing use of antimicrobials in human ,animals and agriculture.
Sensitivity testing is used to select effective antibiotic which is used in
treatment and control of infection especially when caused by pathogens
that are often drug resistant (Schito et al.,2000).
The aim of this study was isolation and identification of E.coli and
enterococcal isolates from urine samples , to determine the antimicrobial
susceptibility for all E.coli and enterococcal isolates using three different
methods of antibiotic sensitivity (disc diffusion, tube dilution and Etest)
and to recommend the best method.
This study was conducted on 80 patients , Their age ranged from
20 to 60 years. They were 42 females (52.5 %) and 38 males (47.5%). 62
cases (77.5%) were outpatients while 18 cases(22.5%)were admitted in
urology department in Benha University Hospital .
Antimicrobial susceptibility test by disc diffusion method was done
for all E.coli isolates to different antimicrobials (ampicillin , cefotaxime ,
ciprofloxacin , chloramphenicol , gentamycin , cephalexin) and revealed
that that the most sensitive antibiotic is gentamycin (93.75%) followed by
ciprofloxacin(56.25%) while the least sensitive antibiotic is
ampicillin(25%).
Summary and Conclusion
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Antimicrobial susceptibility test by disc diffusion method was
also done for all enterococcal isolates to different antimicrobials
(ampicillin, cefotaxime,ciprofloxacin, chloramphenicol, gentamycin,
cephalexin) and revealed that the most sensitive antibiotic is gentamycin
(80%) followed by ciprofloxacin(70%) while the least sensitive antibiotic
is cephalexin(20%).
Disc diffusion ,tube dilution and E-test were used to determine
sensitivity of E.coli and enterococcal isolates to cefotaxime and
ciprofloxacin.
E-test is better than disc diffusion as testing method of sensitivity
of E.coli to cefotaxime and ciprofloxacin. This is done in comparison to
tube dilution method which is taken as reference method.
E-test is better than disc diffusion as testing method of sensitivity
of enterococcal isolates to ciprofloxacin. This is done in comparison to
tube dilution method which is taken as reference method.
Disc diffusion is better than E-test as testing method of sensitivity
of enterococcal isolates to cefotaxime . This is done in comparison to
tube dilution method which is taken as reference method. |
