You are in:Home/Publications/Environmental Mycobacterium avium subsp. hominissuis have a higher probability to act as a recipient in conjugation than clinical strains

Ass. Lect. Salma Abdelrahim Saad Mohamed Shoulah :: Publications:

Title:
Environmental Mycobacterium avium subsp. hominissuis have a higher probability to act as a recipient in conjugation than clinical strains
Authors: Salma A. Shoulah, Anna M. Oschmann, Abdelfattah Selim, Torsten Semmler, Carsten Schwarz, Elisabeth Kamal, Faysal Hamouda, Elsayed Galila, Wilbert Bitter, Astrid Lewin
Year: 2018
Keywords: Conjugation Mycobacterium avium Plasmid Type I restriction modification Antibiotic resistance
Journal: Plasmid
Volume: 95
Issue: Not Available
Pages: 28-35
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Salma Abdelrahim Saad Mohamed Shoulah_Salma plasmid journal.pdf
Supplementary materials Not Available
Abstract:

Mycobacterium avium subsp. hominissuis (MAH) is a widespread opportunistic pathogen that can be isolated from environment (dust, soil and water) and patients with lung or lymphnode infection. In our previous research we revealed the pronounced genetic diversity in MAH by identifying eight different types of a newly described genomic island. In order to identify mechanisms of such horizontal gene transfer we now analyzed the ability of 47 MAH isolates to inherit the conjugative plasmid pRAW from M. marinum. A higher percentage of environmental isolates (22.7%) compared to clinical isolates (8%) had the capacity to function as recipient in conjugal plasmid transfer. Genetic analysis showed additionally that environmental isolates contained more genes homologous to genes present on conjugative mycobacterial plasmids than clinical isolates. Comparative analysis of the genomes of the isolates pointed to a possible association between the ability to act as recipient in conjugation and the structure of a genomic region containing the radC gene and a type I restriction/modification system. Finally we found that uptake of pRAW decreased the resistance against various antibiotics.

Google ScholarAcdemia.eduResearch GateLinkedinFacebookTwitterGoogle PlusYoutubeWordpressInstagramMendeleyZoteroEvernoteORCIDScopus