Background: Extended-spectrum beta-lactamases (ESBLs) are rapidly evolving group of
beta-lactamase enzymes commonly produced by the Gram negative bacteria. Objectives:
This work aimed to detect the bacterial pathogens that cause urinary tract infection and
to detect ESBL producing bacteria among them in Pediatric Department at Benha
University Hospital. Methodology: This study was conducted on 118 children patients
suffering from UTI collected from Outpatient and Inpatient of pediatric department at
Benha University during the period from January 2015 to the end of July 2015. They
were 77 girls and 41 boys and their ages ranged from 1 to 13 years.118 Urine samples
were subjected to isolation and identification of causative organisms, screened for ESBL
production by the pattern of antimicrobial susceptibilities. Phenotypic identification for
ESBL production was confirmed by double disc diffusion test, bla SHV and bla CTX-M
genes in ESBL, producing isolates were detected by using multiplex PCR. Results: This
study showed that the most common cause of children UTI was E.coli (55.1%) followed
by klebsiella pneumoniae (21.2%). Thirty seven (31.4%) of 118 bacterial isolates that
tested by double disc confirmatory test were ESBL positive. Among the ESBL producing
bacteria, 16 out of 37 (43.24%) have CTX-m and SHV genes together while 3 out of 37
(8.1%) have SHV gene only. Conclusion: Our findings showed that majority of ESBL
isolates were positive for both CTXm and SHV genes (43.24%) but SHV gene alone was
positive only in (8.1%) of isolates, So studies should be done in different regions of Egypt
to find the common ESBL genes present in that geographical area for epidemiological
purposes. Also ESBL producing isolates have been increasingly recognized in hospitals
in Egypt and are associated with multiple drug resistance. Thus, ESBL producing
organisms should be identified quickly so that appropriate antibiotic usage and infection
control measures can be implemented.
INTRODUCTION
Urinary tract infection is a serious health problem
affecting children each year. Infections of the urinary
tract are the second most common type of infection after
the infection of the respiratory tract 1.Acute UTIs are
relatively common in children. By seven years of age, 8
% of girls and 2 % of boys will have at least one
episode 2. Baseline abnormalities of the urogenital tract
have been reported in up to 3.2 % of healthy, screened
infants3.Common uropathogens are Escherichia coli,
Klebsiella pneumoniae, Proteus mirabilis,
Enterobacter, Citrobacter Freundi, Staphylococus
saprophyticus, and Enterococci 4 .
*Corresponding author:
Dina S. Ramadn
Department of Microbiology, Faculty of science -Benha University
In older kids, UTIs may cause obvious symptoms |