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Ass. Lect. Tamer Ali :: Experience:

Experience
  • Bioconductor tools.
  • ChIP-Seq (chromatin immunoprecipitation followed by sequencing).
  • RNA-Seq (whole transcriptome sequencing).
  • Proteomics: SILAC and free label detection.
  • Embryonic, adult stem cells and iPSCs biology.
  • Drosophila chromatin insulators.
  • CRISPR/Cas system for genome editing.
  • Chromosome conformation capture to study genome-wide interactions.
  • Basic molecular biology techniques: Cloning (using restriction enzyme cloning strategy and Gibson assembly), site-directed mutagenesis, different types of PCR, western blot, BAC recombineering and generating stable cell lines.
  • Gene expression tuning via RNA-guided dCas9.
  • Cell signaling pathways.
  • lncRNA biology
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