Brucellosis is an important zoonotic disease caused by gram- negative bacteria that is pathogenic for a wide variety of animals and human. The aim of this study was to assess perception of farmers regarding Brucellosis at Kalyobia Governorate. Research design: A descriptive research design was used in carrying out this study. Setting: The study was conducted in sixteen (16) veterinary health units at Kalyobia Governorate. The sample: A simple random sample technique was used to select 320 farmers indirect contact with animals from total (3200). Tools: A structured interviewing questionnaire to assess the socio-demographic characteristics of the studied sample, family history of Brucellosis infection, their knowledge and attitude regarding Brucellosis and observational checklist to assess practices and home environment Results: 79.4% of studied sample were males, 43.4% aged from 30 to less than 40, and 31.6 % of them were illiterate. 77.2% of studied sample had poor knowledge, 69.4% of them had indifferent attitude while 51.2% of them had unsatisfactory practices regarding Brucellosis. Conclusion: Slightly more than three quarters of the studied sample had poor knowledge and more than two thirds had indifferent attitude while slightly more than half of the studied sample had unsatisfactory practice regarding Brucellosis. There were a highly statistically significant correlation between total knowledge and both total practices and total attitudes of the studied sample related to Brucellosis. Recommendations: Health education program should be given for farmers about Brucellosis, its causes, mode of transmission, signs & symptoms, and methods of prevention at veterinary units. Booklet in each veterinary health units.